This is a knockout-validated antibody summary, based on the publication "H3K36me2 recruits DNMT3A and shapes the intergenic DNA methylation landscape", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2bκ
Company: Millipore Sigma
Antibody: WHSC1/NSD2
Catalog number: MABE191
Summary: Mouse monoclonal IgG2bκ against a recombinant protein corresponding to human WHSC1/NSD2. Reacts with human by western blot, immunoprecipitation and chromatin immunoprecipitation (ChIP).
Western blot
Parental and NSD2-KO mouse mesenchymal stem cells (mMSC).
5% non-fat milk in PBS plus 0.5% Tween-20.
Horseradish peroxidase-conjugated anti-mouse secondary antibodies (GE Healthcare).
Immunoblots of lysates from parental and H3K36 methyltransferase knockout
mMSC clonal lines for NSD1, NSD2, and SETD2. Vinculin was used as a loading control.
Please see Extended Data Figure 4a in the article [1].
The same clone (29D1) is sold as Abcam ab75359; Active Motif 39879; EMD Millipore MABE191.
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