This is a knockout-validated antibody summary, based on the publication "A Genetic Screen Identifies a Critical Role for the WDR81-WDR91 Complex in the Trafficking and Degradation of Tetherin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. WDR81 expression was assessed by immunoblot in HeLa cell clones ± Vpu stably expressing Cas9 and two independent WDR81 specific sgRNAs. Fatty acid synthase was used as loading control. From [1].

Rabbit polyclonal IgG

Company: Abcam

Antibody: WDR81

Catalog number: ab121733

Summary: Rabbit polyclonal IgG against an epitope corresponding to amino acids 465-589 of human WDR81. Reacts with human by western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin).

Western blot

Control and WDR81-KO HeLa cells. Cell were lysed in 1% Triton X‐100 in TBS with 0.5 mm phenylmethylsulfonyl fluoride (PMSF), 10 mm iodoacetamide (IAA), and Roche complete protease inhibitor for 30 min on ice.

5% milk (Marvel) in PBS/0.2% Tween.

Goat anti‐rabbit‐HRP (Jackson ImmunoResearch, 111‐035‐144).

SuperSignal West Pico chemiluminescent substrate (Thermo Fisher Scientific) and X‐ray films.