WASp antibody | knockout validation | Santa Cruz sc-13139
DOI
//dx.doi.org/10.13070/ko.en.7.2130
Date
2017-02-15

This is a knockout-validated antibody summary, based on the publication "Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

WASp antibody | knockout validation | Santa Cruz sc-13139 figure 1
Figure 1. Western blot of WASp in CD11c+ or CD8+ dendritic cells obtained from spleen of WT, WASpfl/flCD11cCre/wt, WASpfl/fl and WASp KO (WKO) mice. GAPDH served as loading control. From [1].
Antibody information

Mouse monoclonal IgG2a

Company: Santa Cruz

Antibody: WASp

Catalog number: sc-13139

Summary: Mouse monoclonal IgG2a against amino acids 1-250 of human WASP. Recommended for detection of WASP and N-WASP of mouse, rat, human and mink origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA.

Validation Method

Western blot

Sample

CD11c+ or CD8+ dendritic cells obtained from spleen of WT, WASpfl/flCD11cCre/wt, WASpfl/fl and WASp KO (WKO) mice.

References
  1. Baptista M, Keszei M, Oliveira M, Sunahara K, Andersson J, Dahlberg C, et al. Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells. Nat Commun. 2016;7:12175 pubmed publisher