WASP antibody | knockout validation | Santa Cruz sc-365859

This is a knockout-validated antibody summary, based on the publication "A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: WASP

Catalog number: sc-365859

Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 197-222 within an internal region of human WASP. Recommended for detection of WASP of mouse, rat and human by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including canine.

Validation Method

Western blot

Sample

Peripheral blood T cell lysates from a healthy control and a WASpnull patient and mouse splenic T cells from WT and Was–/– mice.

Figure

Representative immunoblot of the indicated proteins in peripheral blood T cell lysates from a healthy control (HC) and a WASpnull patient (A) and of mouse splenic T cells from WT and Was–/– mice (B). Please see Figure 2a,b in the article [1].

References
  1. Janssen E, Tohme M, Hedayat M, Leick M, Kumari S, Ramesh N, et al. A DOCK8-WIP-WASp complex links T cell receptors to the actin cytoskeleton. J Clin Invest. 2016;: pubmed publisher