WAPL antibody | knockout validation | Santa Cruz sc-365189
This is a knockout-validated antibody summary, based on the publication "The Cohesin Release Factor WAPL Restricts Chromatin Loop Extension", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Figure 1. Western blot analysis of wild-type and ΔWAPL cells. WAPL siRNA-transfected cells are included as a control. From [1].
Antibody information
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: WAPL
Catalog number: sc-365189
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 1-300 mapping at the N-terminus of human WAPL. Recommended for detection of WAPL of human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Validation Method
Western blot
Sample
Wild-type and WAPL-KO HAP1 cells.
Primary incubation
1:1,000 dilution.
Secondary incubation
1:2,000 dilution goat anti-Mouse-PO (DAKO).
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