This is a knockout-validated antibody summary, based on the publication "VDR activation attenuate cisplatin induced AKI by inhibiting ferroptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blots analysis of GPX4 and VDR in renal tissue lysates from these four groups. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Vitamin D Receptor

Catalog number: ab109234

Summary: Rabbit monoclonal IgG against a synthetic peptide within Human Vitamin D Receptor (aa 1-100). Reacts with mouse, rat and human. Suitable for western blot, immunoprecipitation, and chromatin IP. Unsuitable for flow cytometry, immunocytochemistry/immunofluorescence or immunohistochemistry (paraffin).

Western blot

Wild type and VDR-KO mice renal tissues.

WB: 0.1% (w/v) BSA solution on a shaker for 2 h.

WB: at 4 °C for one night.

WB: Fluorescent secondary antibody for 1 h.