This is a knockout-validated antibody summary, based on two publications "Elucidation of GlcNAc-binding properties of type III intermediate filament proteins, using GlcNAc-bearing polymers" (discussed below) [1] and "Vimentin intermediate filaments function as a physical barrier during intracellular trafficking of caveolin-1" (western blot, figure 4a from [2] ). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting analysis of vimentin expression in WT HeLa cells (1 and 2) and vimentin-deficient HeLa cells (3 and 4). M, molecular weight marker, in kDa. Courtesy from the Authors [1].

Mouse monoclonal IgG1

Company: Sigma

Antibody: Vimentin

Catalog number: V6630

Summary: Mouse monoclonal IgG1 against pig eye lens vimentin. Reacts with horse, monkey, bovine, pig, rabbit, canine, gerbil, rat, chicken, feline, human, and hamster. Suitable for western blot and immunohistochemistry (paraffin).

Western blot

WT and vimentin-KO HeLa cells. Total cell lysate was prepared by lysis buffer (1% Triton-X 100, 20 mM HEPES-NaOH, 500 mM NaCl and proteinase and phosphatase inhibitor cocktail (Nacalai Tesque).

The same clone (V9) is sold as Novus Biologicals NBP1-97670; OriGene TA327753; Santa Cruz Biotechnology sc-6260, sc-6260 AC; LifeSpan Biosciences LS-C95348, LS-C87666, LS-C122541, LS-B7192, LS-C312208, LS-C348628; Abnova MAB3578, MAB16241; Abcam ab8069; Invitrogen 11-9897-80, 11-9897-82, 14-9897-80, 14-9897-82, 42-9897-82, MA5-11880, MA5-11883, 41-9897-80, 41-9897-82, 50-9897-80, 50-9897-82, MA5-11883-A488, MA5-11883-A555, MA5-11883-A647; MyBioSource MBS210577, MBS370116; Boster MA1102; Dako M0725; Sigma-Aldrich V6630, V6389, C9080; Cell Sciences MON3005-1.