This is a knockout-validated antibody summary, based on the publication "Selective regulation of Notch ligands during angiogenesis is mediated by vimentin", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot and coimmunoprecipitation of vimentin and Jagged1 from VimWT and VimKO MEFs. Courtesy from the Authors [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Vimentin

Catalog number: 5741

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Arg45 of human vimentin protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunohistochemistry (paraffin), immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

WT and Vim-KO MEFs.

5% nonfat dry milk at room temperature (RT) for 1 h.

Overnight at 4 °C

Horseradish peroxidase-conjugated secondary for 1 h at RT.

Western Lightning Plus-ECL Enhanced chemiluminescence substrate (Perkin-Elmer).