This is a knockout-validated antibody summary, based on the publication "PCP Signaling between Migrating Neurons and their Planar-Polarized Neuroepithelial Environment Controls Filopodial Dynamics and Directional Migration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Dorsal view of wild type (A) and vangl2 mutant (B) 24 hpf neural tubes immunostained with anti-Vangl2-NT (green). The neuroepithelial membrane staining visible in wild type is absent in the mutant. Western blot analysis of whole embryo lysates with anti-Vangl2 antibody. Anti-alpha-tubulin was used as a loading control. Zebrafish Vangl2 is expected to run at approximately 60kDa. For the anti-Vangl2 blot there is a band that is present in the wild type and absent in the vangl2 mutant, see asterisk. From [1].

Rabbit polyclonal

Company: Anaspec

Antibody: Vangl2

Catalog number: AS-55659

Summary: Rabbit polyclonal against synthetic peptide derived from the N-terminal region of zebrafish Vangl-2 protein. It reacts with zebrafish.. Suitable for western blot, immunohistochemistry (whole-mount) and ELISA.

Western blot | Immunohistochemistry

WB: whole embryo zebrafish lysates.

IHC: Zebrafish embryos brain tissue.

1:250 dilution.

IHC: Zeiss 700 confocal microscope

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.