This is a knockout-validated antibody summary, based on the publication "Generation and Characterisation of a Pax8-CreERT2 Transgenic Line and a Slc22a6-CreERT2 Knock-In Line for Inducible and Specific Genetic Manipulation of Renal Tubular Epithelial Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Histological images of representative renal sections from 12 month old control, Pax8-CreERT2/VhlΔ/Δ and Slc22a6-CreERT2/VhlΔ/Δ mice stained with VHL antibody. Scale bars, 100 um. From [1].

Antibody: VHL

Catalog number: sc-5575

Summary: Rabbit polyclonal IgG raised against the epitope corresponding to amino acids 1-181 representing full length von Hippel-Lindau (VHL) tumor suppressor protein of mouse origin. Recommended for detection of VHL of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Company: Santa Cruz

IHC (either frozen or formalin fixed)

Renal sections from 12 month old control, Pax8-CreERT2/VhlΔ/Δ and Slc22a6-CreERT2/VhlΔ/Δ mice

Dilution 1:200

Secondary antibodies conjugated to HRP

Developed with DAB

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.