This is a knockout-validated antibody summary, based on the publication "A non-canonical Notch complex regulates adherens junctions and vascular barrier function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: VE-cadherin
Catalog number: sc-9989
Summary: Mouse monoclonal IgG1 (kappa light chain) specific for an epitope mapping between amino acids 768-784 at the C-terminus of human VE-cadherin. Recommended for detection of VE-cadherin of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, flow cytometry and ELISA. Also reactive with additional species, including porcine.
Immunocytochemistry
Control and VE-cadherin knockout human dermal microvascular endothelial cells (ECs).
1:200 dilution.
HRP-conjugated donkey anti-mouse IgG (Fitzgerald).
Chemiluminescent HRP detection.
Fluorescent micrographs of TMD-mApple expressed in VE-cadherin knockout or scramble control ECs and immunostained for VE-cadherin. Please see Extended Data Figure 6c in the article [1].
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