VDR antibody | knockout validation | Santa Cruz sc-13133
DOI
//dx.doi.org/10.13070/ko.en.7.2117
Date
2017-02-15

This is a knockout-validated antibody summary, based on the publication "Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

VDR antibody | knockout validation | Santa Cruz sc-13133 figure 1
Figure 1. Immunofluorescence histochemistry of VDR in WT and VDR-KO mouse small intestine and muscle. From [1].
Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: VDR

Catalog number: sc-13133

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 344-424 of human VDR. Recommended for detection of VDR of human, mouse and rat by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Validation Method

immunohistochemistry

Sample

Cryosections of small intestine and muscle from VDR-/- and wild type mice.

Blocking agent

1% BSA.

Primary incubation

Overnight.

Detection

Fluorescence microscope (Leica, DMIL/DFC450).

References
  1. Zhang T, Yin Y, Liu H, Du W, Ren C, Wang L, et al. Generation of VDR Knock-Out Mice via Zygote Injection of CRISPR/Cas9 System. PLoS ONE. 2016;11:e0163551 pubmed publisher