This is a knockout-validated antibody summary, based on the publication "MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Vdac2 in WT MEF, vdac2−/− MEF, and the 3xFLAG-VDAC2 rescued vdac2−/− MEF. Beta-actin (ACTB) served as loading control. From [1].

Goat polyclonal

Company: Abcam

Antibody: VDAC2

Catalog number: ab37985

Summary: Goat polyclonal raised against a synthetic peptide:

corresponding to C terminal amino acids 283-294 of Human VDAC2. Reacts with mouse, rat, and human and is predicted to work with rabbit, cow, dog, and pig. Suitable for western blot and immunocytochemistry.

Western blot

vdac2−/− MEFs

overnight at 4°C

peroxidase-conjugated AffiniPure goat anti-mouse IgG, light chain* specific (Jackson ImmunoResearch Laboratories, 115-035-174), 1 h at room temperature

Super Signal Chemiluminescent Substrate system (Pierce, Rockford, 34080)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.