This is a knockout-validated antibody summary, based on the publication "Vasodilator-Stimulated Phosphoprotein (VASP)-dependent and -independent pathways regulate thrombin-induced activation of Rap1b in platelets", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Platelets from wild type (WT) or VASP-null platelets (VASP KO) mice. Total Rap1b serves as loading control. From [1].

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: VASP

Catalog number: sc-46668

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 271-360 of human VASP. Recommended for detection of VASP of human, mouse and rat origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot.

Platelets from wild type or VASP-null (VASP KO) mice. Platelets were lysed with an ice cold buffer composed of 50 mM Tris-HCl (pH 7.4), 1 % Triton X- 100, 100 mM NaCl, 10 mM MgCl2, 20 % glycerol, 1 mM Na3VO4, and 1 mM pefabloc, and Rap1b was immunoprecipitated.

Phosphate-buffered saline supplemented with 0.2 % Tween 20 and 3 % milk.

Overnight at 4 °C.

1:10,000 dilution peroxidase-conjugated goat anti-mouse IgGs for 1 h.

Enhanced chemiluminescence.