VASP antibody | knockout validation | Cell Signaling 3132

This is a knockout-validated antibody summary, based on the publication "Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: VASP

Catalog number: 3132

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues near the carboxy terminus of human and mouse VASP. Reacts with human, mouse, rat, hamster, monkey, and bovine. Suitable for western blot, immunoprecipitation, and immunofluorescence/immunocytochemistry.

Validation Method

Western blot

Sample

WT and EVL/VASP dKO murine T cells.

Secondary incubation

Licor donkey anti-rabbit IR800.

Figure

VASP protein is expressed in WT T cells, but is not expressed in EVL/VASP dKO T cells. Please see Figure S1b in the article [1].

References
  1. Estin M, Thompson S, Traxinger B, Fisher M, Friedman R, Jacobelli J. Ena/VASP proteins regulate activated T-cell trafficking by promoting diapedesis during transendothelial migration. Proc Natl Acad Sci U S A. 2017;114:E2901-E2910 pubmed publisher