This is a knockout-validated antibody summary, based on the publication "Regulation of proximal T cell receptor signaling and tolerance induction by deubiquitinase Usp9X", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Usp9X in B and T lymphocytes purified from the spleen of mice having Usp9X specifically deleted in B cells (using CD19.cre) or in all hematopoietic cells (using vavCre transgene). Actin was used as loading control. From [1].

Mouse monoclonal.

Company: Genentech

Antibody: Usp9X

Catalog number: 4B3.1.1

Summary: Info not available on Genentech website.

Western blot

B and T lymphocytes purified from the spleen of mice having Usp9X specifically deleted in B cells (using CD19.cre) or in all hematopoietic cells (using vavCre transgene). Cells were lysed in RIPA lysis buffer (50 mM Tris, pH 7.4, 150 mM NaCl, 2 mM EDTA, 1% NP-40, and 0.1% SDS) supplemented with protease (Roche) and phosphatase inhibitors (Roche).

1:2,500 dilution of HRP-conjugated anti–rabbit IgG secondary (Cell Signaling Technology) or HRP-conjugated anti–rat IgG secondary (Santa Cruz Biotechnology, Inc.)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.