This is a knockout-validated antibody summary, based on the publication "53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblots of extracts from control (Ctrl) and knockout RPE1 lines. Bands corresponding to each protein (arrowheads) and nonspecific bands (asterisks) are indicated. α-Tubulin serves as a loading control. From [1].

Rabbit monoclonal IgG

Company: abcam

Antibody: Usp28

Catalog number: ab126604

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues in human USP28. Reacts with human by western blot and immunocytochemistry/immunofluorescence. Unsuitable for immunoprecipitation, immunohistochemistry (paraffin) or flow cytometry.

Western blot

Control and Usp28-knockout RPE1 cell lines. Cells were lysed by sonication in RIPA buffer plus protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific).

TBS-T plus 5% nonfat dry milk or in TBS-T plus 5% BSA.

1:1,000 dilution in blocking solution.

HRP-conjugated secondary antibodies (GE Healthcare).

WesternBright Sirius (Advansta) or SuperSignal West Femto (Thermo Fisher Scientific) substrates. Membranes were imaged on a ChemiDoc MP system (Bio-Rad

Laboratories).