This is a knockout-examination antibody summary, based on the publication "Usp22 deficiency impairs intestinal epithelial lineage specification in vivo", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Usp22 protein levels in brain and small intestine in wildtype and Usp22lacZ/lacZ mice. GAPDH serves as loading control. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Usp22

Catalog number: sc-390585

Summary: Mouse monoclonal IgG1 (kappa light chain) raised against amino acids 130-176 mapping within an internal region of USP22 of human origin. Recommended for detection of USP22 of mouse, rat and human origin by western, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine, canine and bovine.

Western blot

Mouse Usp22lacZ/lacZ brain and small intestine, lysed in RIPA buffer (1% NP-40, 0.5% sodium deoxycholate and 0.1% SDS in PBS) containing 1 mm N-ethylmaleimide, 1 mm Pefabloc and 1 μg/ml Aprotinin/Leupeptin.

horseradish peroxidase-conjugated secondary antibodies (Santa Cruz)

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.