Uhrf1 antibody | knockout validation | Active Motif 61341

This is a knockout-validated antibody summary, based on the publication "UHRF1-repressed 5'-hydroxymethylcytosine Is Essential for the Male Meiotic Prophase I", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Uhrf1 antibody | knockout validation | Active Motif 61341 figure 1
Figure 1. Western blot assay to the 16 dpp mouse testicle tissue showing the knockout efficiency of UHRF1 protein. At least three independent experiments were carried out. From [1].
Antibody information

Mouse monoclonal IgG1k

Company: Active Motif

Antibody: Uhrf1

Catalog number: 61341

Summary: Mouse monoclonal IgG1k against a synthetic peptide corresponding to amino acid residues 263-793 of human Uhrf1. Reacts with human by western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin).

Uhrf1 antibody | knockout validation | Active Motif 61341 figure 2
Figure 2. Immunohistochemical assay to the 16 dpp mouse testicle tissue showing the knockout efficiency of UHRF1 protein. At least three independent experiments were carried out. Scale bar, 25 μm. From [1].
Validation Method

Western blot | Immunohistochemistry

Sample

Control and Uhrf1-cKO mouse testicle tissues.

Primary incubation

WB: 1:1,000 dilution.

IHC: 1:200 dilution overnight at 4 °C.

Secondary incubation

WB; 1:2,000 dilution.

IHC: for 20 min.

References
  1. Pan H, Jiang N, Sun S, Jiang H, Xu J, Jiang X, et al. UHRF1-repressed 5'-hydroxymethylcytosine is essential for the male meiotic prophase I. Cell Death Dis. 2020;11:142 pubmed publisher