Ubc13 antibody | knockout validation | Invitrogen/Zymed 37-1100
DOI
//dx.doi.org/10.13070/ko.en.6.1787
Date
2016-11-08
This is a knockout-validated antibody summary, based on the publication " The human T-cell leukemia virus type 1 Tax oncoprotein requires the ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Figure 1. Western blot of wild-type and Ubc13 -/- murine embryonic fibroblasts probed with anti-Ubc13 antibody (Invitrogen, Cat No. 37-1100). Actin serves as a loading control. From [1].
Antibody information
Mouse monoclonal
Company: Invitrogen
Antibody: Ubc13
Catalog number: 37-1100 Clone 4E11
Summary: Mouse monoclonal antibody raised against recombinant human Ubc1.
Supplier recommended for elisa, IF and WB.
Reacts with human, mouse and rat Ubc13.
Validation Method
Western blot
Sample
Lysates of wild-type and UBC13 -/- murine embryonic fibroblasts.
Blocking agent
5% dried milk powder.
Detection
Western Lightning ECL Reagent (Perkin Elmer).
References
- Shembade N, Harhaj N, Yamamoto M, Akira S, Harhaj E. The human T-cell leukemia virus type 1 Tax oncoprotein requires the ubiquitin-conjugating enzyme Ubc13 for NF-kappaB activation. J Virol. 2007;81:13735-42 pubmed
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