UVRAG antibody | knockout validation | Cell Signaling 13115

This is a knockout-validated antibody summary, based on publications "Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin", as cited below [1], and "Transglutaminase 3 crosslinks the secreted gel-forming mucus component Mucin-2 and stabilizes the colonic mucus layer" for immunohistochemistry knockout validation (figure 1c) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

UVRAG antibody | knockout validation | Cell Signaling 13115 figure 1
Figure 1. Western blot analysis of UVRAG in MCF7.control and MCF7.beclin 1 cells transduced with a CRISPR/Cas9 lentivirus containing either no sgRNA (Empty) or two sgRNAs targeting the indicated gene. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: UVRAG

Catalog number: 13115

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Gly502 of human UVRAG protein. Reacts with human and mouse. It is predicted to react based on 100% sequence homology with monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and UVRAG KO MCF7 cells.

Primary incubation

1:2,000 dilution.

References
  1. Wijshake T, Zou Z, Chen B, Zhong L, Xiao G, Xie Y, et al. Tumor-suppressor function of Beclin 1 in breast cancer cells requires E-cadherin. Proc Natl Acad Sci U S A. 2021;118: pubmed publisher
  2. Sharpen J, Dolan B, Nystr xf6 m E, Birchenough G, Arike L, Martínez Abad B, et al. Transglutaminase 3 crosslinks the secreted gel-forming mucus component Mucin-2 and stabilizes the colonic mucus layer. Nat Commun. 2022;13:45 pubmed publisher