UTY antibody | knockout validation | Abcam ab91236
DOI
//dx.doi.org/10.13070/ko.en.7.2183
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "Target sequencing and CRISPR/Cas editing reveal simultaneous loss of UTX and UTY in urothelial bladder cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

UTY antibody | knockout validation | Abcam ab91236 figure 1
Figure 1. Western blotting assay for validating target protein expression in UTX and UTY knockout cells. GAPDH was used as loading control. [1].
Antibody information

Mouse monoclonal IgG1

Company: Abcam

Antibody: UTY

Catalog number: ab91236

Summary: Mouse monoclonal IgG1 against recombinant fragment of human UTY. Reacts with human and mouse. Suitable for western blot, immunoprecipitation and ELISA.

Validation Method

Western blot

Sample

WT and UTY-knockout HT-1197 and UMUC3 cell lines.

Primary incubation

1:1,000 dilution.

Secondary incubation

1:1,000 dilution rabbit-anti-mouse HRP antibody (Abcam, ab6728).

Detection

Clarity™ Western ECL Substrate (Bio-Rad, USA).

References
  1. Ahn J, Kim K, Park S, Ahn Y, Kim H, Yoon H, et al. Target sequencing and CRISPR/Cas editing reveal simultaneous loss of UTX and UTY in urothelial bladder cancer. Oncotarget. 2016;: pubmed publisher