This is a knockout-validated antibody summary, based on the publication "Loss of KDM6A Activates Super-Enhancers to Induce Gender-Specific Squamous-like Pancreatic Cancer and Confers Sensitivity to BET Inhibitors", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: UTX
Catalog number: 33510
Summary: Rabbit monoclonal IgG against a recombinant protein surrounding Ala490 of human UTX protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation and immunohistochemistry (paraffin).
Western blot
Primary murine KrasG12D; Kdm6a null pancreatic cancer cell lines. Cells were solubilized in lysis buffer (25mM Tris-HCl pH 7.6, 200 mM NaCl, 1 mM EDTA, 1% Igepal CA-630, 0.1% Na-Deoxycholate, 0.1% SDS supplemented with a mixture of protease inhibitors).
Western blots showing the expression of ΔNp63 isoform in Kdm6a null females and a male cell line with concomitant loss of Uty. Please see Figure 3d in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.