USP9X antibody | knockout validation | Sigma WH0008239M1

This is a knockout-validated antibody summary, based on the publication "USP9X regulates centrosome duplication and promotes breast carcinogenesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

USP9X antibody | knockout validation | Sigma WH0008239M1 figure 1
Figure 1. Wild type and USP9X knockout HMEC cells were transfected with control vector, FLAGUSP9X/wt or FLAG-USP9X/C1566S. From [1].
Antibody information

Mouse monoclonal IgG1κ

Company: Sigma

Antibody: USP9X

Catalog number: WH0008239M1

Summary: Mouse monoclonal IgG1κ against a partial recombinant protein USP9X (a.a. 1-91). Reacts with human protein by western blot, immunofluorescence and ELISA.

Validation Method

Western blot

Sample

Wild type and USP9X knockout HMEC cells.

Clone note

The same clone (1C4) is sold as Bio-Rad MCA4226Z; Abnova H00008239-M01; Sigma-Aldrich WH0008239M1.

References
  1. Li X, Song N, Liu L, Liu X, Ding X, Song X, et al. USP9X regulates centrosome duplication and promotes breast carcinogenesis.. Nat Commun. 2017;8:14866 pubmed publisher