This is a knockout-validated antibody summary, based on the publication "Proteotoxic stress induces phosphorylation of p62/SQSTM1 by ULK1 to regulate selective autophagic clearance of protein aggregates", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot analysis of WT and ULK1 KO MEFs lysates with anti-ULK1 and actin (loading control) antibodies. Asterisks indicate non-specific bands. From [1].

Rabbit polyclonal

Company: Sigma-Aldrich

Antibody: ULK1

Catalog number: A7481

Summary: Rabbit polyclonal against a synthetic peptide corresponding to amino acids 415-430 of mouse Atg1/ULK1 (conjugated to KLH). Reacts with mouse and rat. Is predicted to react with human since the corresponding sequence differs by 3 amino acids. Suitable for western blot.

Western blot

WT and ULK1 KO MEFs. Cells were lysed in RIPA buffer(50 mM Tris-HCl pH 7.4, 1% NP-40, 0.25% Na-deoxycholate, 150 mM NaCl, 1 mM EDTA) containing complete protease inhibitor cocktail, and phosphatase inhibitor cocktail for 30 min at 4°C.

Overnight at 4°C.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.