This is a knockout-validated antibody summary, based on the publication "TBK1 at the Crossroads of Inflammation and Energy Homeostasis in Adipose Tissue", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: ULK1
Catalog number: 8054
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Arg600 of human ULK1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.
Western blot
Epididymal white adipose tissue (WAT) from FL/FL and ULK1 adipocyte-specific KO mice. Tissues or cells were homogenized in a non-denaturing lysis buffer that contained 10 mM Tris (pH 7.4), 150 mM NaCl, 1 mM EGTA, 1 mM EDTA, 1% Triton X-100, 0.5% Nonidet P-40, with freshly added protease inhibitors tablet (Roche), and phosphatase inhibitor 2 mM sodium vanadate and 5 mM sodium fluoride.
Horseradish peroxidase-conjugated secondary antibodies (Fisher Scientific).
SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific).
Immunoblot analysis of epididymal WAT from FL/FL and ULK1 adipocyte-specific KO mice after the indicated treatments. Please see Figure 4f in the article [1].
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