This is a knockout-validated antibody summary, based on the publication "Flow Cytometry of Mouse and Human Adipocytes for the Analysis of Browning and Cellular Heterogeneity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative images of unsorted BAT adipocytes from untreated or CL 316,243-treated WT or UCP1-KO mice stained with isotype control or anti-UCP1 antibody (red). Intact cells are co-stained with Syto-12 (green). Note the complete lack of UCP1 staining in the knockout sample. Scale bars, 50 μm. From [1].

Mouse monoclonal IgG2B

Company: R&D Systems

Antibody: UCP1

Catalog number: MAB6158

Summary: Mouse monoclonal IgG2B against recombinant human UCP1

Met1-Thr307. Reacts with mouse and human. Suitable for western blot, immunocytochemistry and flow cytometry.

Immunocytochemistry

BAT adipocytes from WT or UCP1-KO mice.

3% normal donkey serum, 2% BSA, 0.25% Triton X-100 in DPBS under gentle agitation.

1:300 dilution for 1 h.

Primary antibody was conjugated to Alexa647 (ThermoFisher Alexa Fluor 647 antibody labeling kit A20186).

Inverted confocal Zeiss LSM 700 or LSM 880 confocal laser scanning microscope using the ZEN software (Carl Zeiss, Germany).