This is a knockout-validated antibody summary, based on the publication "Absence of UCHL 1 function leads to selective motor neuropathy", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. (D) There is no UCHL1 protein in the spinal cord of UCHL1−/− mice by western blot analysis. β‐tubulin is used as loading control. (E) UCHL1 expression cannot be detected in the UCHL1−/− spinal cord by immunocytochemistry. (F) UCHL1 is not expressed in the muscle of WT or UCHL1−/− mice. UCHL1 expression colocalizes with the axons. C: cervical; L: lumbar; Scale bars: E = 200 μm, E enlarged = 50 μm, F = 50 μm. From [1].

Rabbit polyclonal IgG

Company: ProteinTech

Antibody: UCHL1

Catalog number: 14730-1-AP

Summary: Rabbit polyclonal IgG against UCHL1/PGP9.5 fusion protein. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry, and ELISA.

Western blot | Immunohistochemistry

WB: Spinal cords were isolated from WT and UCHL1-/-. Tissue was homogenized in 1 mL of T-PER (Tissue Protein Extraction Reagent, Pierce, Rockford, IL) supplemented with protease inhibitor cocktail (Calbiochem, Billerica, MA). IHC: mouse spinal cords, and soleus and EDL muscle.

WB: 1:2,000 dilution overnight at 4°C.

IHC: 1:1,000 dilution.

WB: HRP conjugated secondary antibodies.

IHC: fluorescent conjugated goat AlexaFluor 488 or Cy3.

WB: Enhanced chemiluminescence (Millipore, Temecula, CA).

IHC: Nikon SMZ1500 and Nikon Eclipse TE2000-E fluorescence microscopes equipped with Intensilight C-HGFI (Nikon Inc., Melville, NY) were used. Epifluorescence images were acquired using a Digital Sight (DS)-Qi1MC CCD camera (Nikon Inc.). Confocal

images were collected using a Zeiss 510 Meta confocal microscope (Carl Zeiss Inc., Thornwood, NY).