This is a knockout-validated antibody summary, based on the publication "Subcellular organization of UBE3A in neurons", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2aκ

Company: Sigma-Aldrich

Antibody: UBE3A

Catalog number: SAB1404508

Summary: Mouse monoclonal IgG2aκ against a partial recombinant protein UBE3A (51 a.a. ~ 150 a.a). Reacts with human by western blot and ELISA.

Immunohistochemistry

Brain sections from WT, Angelman syndrome model and UBE3A KO adult mice.

10% normal donkey serum.

1:10,000 dilution.

1:200 dilution donkey IgG conjugated to DyLight 549 (Jackson ImmunoResearch; West Grove, PA).

Sections were examined with a Zeiss LSM 880 confocal microscope.

UBE3A expression in mouse brain from WT (A, B), AS model (C, D), and KO (E, F) mice. Sagittal sections were immunostained for UBE3A (left column, A, C, E) and counterstained with DAPI (right column, B, D, F). UBE3A staining is markedly diminished in the AS mice, and nearly absent in the KO. Scale bars = 250 µm. Please see Figure 1 in the article [1].

The same clone (3E5) is sold as Abnova H00007337-M02; Sigma-Aldrich SAB1404508.