This is a knockout-validated antibody summary, based on the publication "The Spindle Assembly Checkpoint Is Not Essential for Viability of Human Cells with Genetically Lowered APC/C Activity", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of UBE2S and UBE2C levels in WT, UBE2S- and UBE2C-knockout HCT116 cells. Tubulin is used as loading control, from figure 1 [1].

Antibody: UBE2S

Catalog number: PAB1701

Summary: Rabbit polyclonal antibody against a synthetic peptide (conjugated with KLH) corresponding to C-terminus of human UBE2S. It recognizes endogenous levels of total UBE2S protein from human. Used in western blot and immunohistochemistry.

Company: Abnova

Western blot

UBE2S-knockout HCT116 cells

The author used two UBE2S antibodies, one from Cell Signaling Technology 9630S, which is discontinued, and this PAB1701 antibody. The authors did not specify which antibody was used in this particular figure. There are several western blot using KO cells and we assumed that both were KO validated.