This is a knockout-validated antibody summary, based on the publication "A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of UBE2G2 in control and UBE2G2-knockout 293FT cells. Actin served as loading control. From [1].

Rabbit monoclonal IgG

Company: abcam

Antibody: UBE2G2

Catalog number: ab174296

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Ube2G2 aa 1-100. Reacts with human, mouse, and rat. Suitable for western blot, immunoprecipitation, and immunofluorescence/immunocytochemistry. Unsuitable for flow cytometry and immunohistochemistry (paraffin).

Figure 2. Western blot of UBE2G2 in control and UBE2G2-knockout Neuro-2a cells. Actin served as loading control. From [1].

Western blot

Control and CRISPR-mediated UBE2G2-knockout 293FT, Neuro-2a and HeLa cells. Cells were lysed with RIPA buffer supplemented with protein inhibitor cocktail (Roche Life Science).

5% skim milk in TBST overnight.

Figure 3. Western blot of UBE2G2 in control and UBE2G2-knockout HeLa cells. Actin served as loading control. From [1].

1:1,000 dilution for 2 hours.

HRP-conjugated secondary antibody for 1 hour.

ECL western blotting substrate (Thermo Scientific), according to the manufacturer’s instructions.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.