This is a knockout-validated antibody summary, based on the publication "TSC2 mediates hyperosmotic stress-induced inactivation of mTORC1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Immunoblot of TSC2 in control and TSC2 knock-out MEFs. Tubulin was used as loading control. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling Technology

Antibody: Tuberin/TSC2

Catalog number: 4308

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to carboxy-terminal residues of human tuberin (TSC2) protein. Reacts with human, mouse, rat, hamster, and monkey. Suitable for western blotting, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry.

Western blot

Control and TSC2 knock-out MEFs. Cells were lysed in-well with ice-cold Triton lysis buffer (50 mM Tris pH 7.5, 1% Triton X-100, 150 mM NaCl, 50 mM NaF, 2 mM Na-vanadate, 0.011 gr/ml beta-glycerophosphate, 1× PhosSTOP phosphatase inhibitors and 1× Complete protease inhibitors) for 10 minutes on ice.

1:2,000 dilution.

LICOR Fc detection system.