Tsg101 antibody | knockout validation | Santa Cruz sc-7964
DOI
//dx.doi.org/10.13070/ko.en.7.2126
Date
2017-02-15

This is a knockout-validated antibody summary, based on the publication "A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Tsg101 antibody | knockout validation | Santa Cruz sc-7964 figure 1
Figure 1. Western blot analysis of Tsg101 in floxed (fl/fl), KO (-/-) and rescued (-/- +HA-Tsg101) MEFs. β-actin (ActB) was used as loading control. From [1].
Antibody information

Mouse monoclonal IgG2a (kappa light chain)

Company: Santa Cruz

Antibody: Tsg101

Catalog number: sc-7964

Summary: Mouse monoclonal IgG2a (kappa light chain) against amino acids 1-138 representing full length mouse tsg 101 (tumor susceptibility gene 101). Recommended for detection of tsg 101 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Validation Method

Western blot

Sample

Control and Tsg101-KO mouse embryonic fibroblasts with and without exogenous re-expression of Tsg101.

Primary incubation

1∶1,000 dilution.

Secondary incubation

1∶1,000 dilution horseradish peroxidase-conjugated secondary antibodies (Santa Cruz Biotechnology).

References
  1. Morris C, Stanton M, Manthey K, Oh K, Wagner K. A knockout of the Tsg101 gene leads to decreased expression of ErbB receptor tyrosine kinases and induction of autophagy prior to cell death. PLoS ONE. 2012;7:e34308 pubmed publisher