This is a knockout-validated antibody summary, based on the publication "53BP1 and USP28 mediate p53 activation and G1 arrest after centrosome loss or extended mitotic duration", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblots of extracts from control (Ctrl) and knockout RPE1 lines. Bands corresponding to each protein (arrowheads) and nonspecific bands (asterisks) are indicated. α-Tubulin serves as a loading control. From [1].

Rabbit polyclonal IgG

Company: Bethyl

Antibody: Trim37

Catalog number: A301-174A

Summary: Rabbit polyclonal IgG against an epitope mapping between residues 914 and 964 of human Trim37. Reacts with human protein by western blot and immunoprecipitation.

Western blot

Control and Trim37-knockout RPE1 cell lines. Cells were lysed by sonication in RIPA buffer plus protease and phosphatase inhibitor cocktail (Thermo Fisher Scientific).

TBS-T plus 5% nonfat dry milk or in TBS-T plus 5% BSA.

1:2,000 dilution in blocking solution.

HRP-conjugated secondary antibodies (GE Healthcare).

WesternBright Sirius (Advansta) or SuperSignal West Femto (Thermo Fisher Scientific) substrates. Membranes were imaged on a ChemiDoc MP system (Bio-Rad

Laboratories).