Topoisomerase I antibody | knockout validation

Topoisomerase I antibody | knockout validation | Abcam ab109374

This is a knockout-validated antibody summary, based on the publication "Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Topoisomerase I antibody | knockout validation | Abcam ab109374 figure 1

Figure 1. TOP1 was completely deleted in TOP1 KO cells through CRISPR/Cas9 approach. β-actin was used as a loading control. From [1].

Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: Topoisomerase I

Catalog number: ab109374

Summary: Rabbit monoclonal antibody against a synthetic peptide of TOP1. Rects with mouse, rat and human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and flow cytometry (intra). Unsuitable for immunoprecipitation.

Validation Method

Western blot

Sample

Control and TOP1-KO Huh7 and PLC/PRF/5 (PLC) cells.

References

Gu Y, Wang Y, He L, Zhang J, Zhu X, Liu N, et al. Circular RNA circIPO11 drives self-renewal of liver cancer initiating cells via Hedgehog signaling. Mol Cancer. 2021;20:132 pubmed publisher

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