This is a knockout-validated antibody summary, based on the publication "Method for Dual Viral Vector Mediated CRISPR-Cas9 Gene Disruption in Primary Human Endothelial Cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Protein expression of Tie2 in vector control and Tie2 knockout HLMVECs induced by CRISPR-Cas9 was determined by immunoblotting. Overexpression of mouse Tie2 in Tie2−/− HLMVECs was determined to assess the ability to rescue Tie2 expression after deletion. From [1].

Mouse monoclonal IgG1κ

Company: Millipore

Antibody: Tie2/TEK

Catalog number: 05-584

Summary: Mouse monoclonal IgG1κ against a fusion protein corresponding to residues 1-745 of human Tie2/TEK. Reacts with human, mouse, rat and pig. Suitable for western blot, immunoprecipitation and immunohistochemistry (frozen). This antibody is not suitable for paraffin-embedded sections.

Western blot

Control and Tie2 knockout primary human lung microvascular endothelial cells (HLMVECs).

ImageQuant LAS 4000 (GE Healthcare).

The same clone (Ab33) is sold as Cell Signaling Technology 4224; EMD Millipore 05-584, FCMAB404PE.