This is a knockout-validated antibody summary, based on the publication "Effects of thrombospondin-4 on Pro-Inflammatory Phenotype Differentiation and Apoptosis in Macrophages", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting with anti-TSP-4 and anti-β-actin antibodies in cultured BMDM isolated from WT, TSP-4-KO and P387-TSP-4-KI mice. From [1].

Mouse monoclonal IgM (kappa light chain)

Company: Santa Cruz

Antibody: Thrombospondin 4

Catalog number: sc-390734

Summary: Mouse monoclonal IgM (kappa light chain) specific for an epitope mapping between amino acids 930-961 of human Thrombospondin 4. Recommended for detection of Thrombospondin 4 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and ELISA.

Western blot

WT and Thbs4−/− bone-marrow-derived macrophages (BMDM). Cells were harvested and lysed on ice using RIPA lysis and extraction buffer (# 89901, Thermo Scientific) supplemented with Halt™ protease and phosphatase inhibitor cocktail (#78442, Thermo Scientific).

5% skim milk in TBST for an hour at room temperature.

1:100 dilution overnight at 4 °C.

HRP anti-mouse secondary antibodies (#sc-516102, Santa Cruz Biotechnology).

Pierce® ECL western blotting substrate on X-ray films.