This is a knockout-validated antibody summary, based on the publications "HIF-2α-mediated induction of pulmonary thrombospondin-1 contributes to hypoxia-driven vascular remodelling and vasoconstriction", as cited below [1] and "Lysyl hydroxylase 1 (LH1) deficiency promotes angiotensin II (Ang II)-induced dissecting abdominal aortic aneurysm" for immunohistochemistry knockout validation (figure 3l) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Protein levels in lung samples from WT and tsp1−/− mice challenged with normoxia (Nx) or hypoxia (Hp) were detected via western blot probed against TSP1, HIF-2α, and α-Tubulin as a loading control. From [1].

Mouse monoclonal IgG1

Company: Invitrogen

Antibody: Thrombospondin 1

Catalog number: MA5-13398, 39-9300

Summary: Mouse monoclonal IgG1 against reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets. Reacts with bovine, dog, horse, human, mouse, sheep, pig, and rat. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin), and flow cytometry.

Western blot

Lungs from WT and TSP1 KO mice. Lysates of snap-frozen tissues and isolated murine

pulmonary cells were prepared in RIPA buffer (50 mM TRIS (pH 7.5), 1% NP-40, 1 mM EDTA, 125 mM NaCl, 0.25% sodium deoxycholate, 1 mM sodium orthovanadate, 1 mM sodium fluoride, and 1× phosphatase/protease inhibitors cocktail (Roche Applied Science, Hercules, CA, USA)).

HRP-conjugated secondary antibodies.

HRP substrate (Luminata Forte, Millipore, Madrid, Spain) on ImageQuant LAS 4000 (GE Healthcare Life Sciences, Madrid, Spain).

The same clone (A6.1) is sold as Novus Biologicals NB100-2059; OriGene DM315, DM315-05; Santa Cruz Biotechnology sc-59887; LifeSpan Biosciences LS-B2570, LS-C87484, LS-C87485, LS-C95513; Abcam ab1823; Invitrogen MA5-13395, MA5-13398, 39-9300, 14-9756-80, 14-9756-82.