This is a knockout-validated antibody summary, based on the publication "Doubly Phosphorylated Peptide Vaccines to Protect Transgenic P301S Mice against Alzheimer’s Disease Like Tau Aggregation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of Tfr1 protein in Tfr1fl/fl control and Tfr1IEC-KO mice. β-actin was used as loading control. From [1].

Mouse monoclonal IgG1κ

Company: Life Technologies

Antibody: Tfr1

Catalog number: 13-6890

Summary: Mouse monoclonal IgG1κ against recombinant human transferrin receptor. Reacts with human, mouse, rat, hamster, and chicken. Suitable for western blot, immunoprecipitation, immunohistochemistry, immunofluorescence, flow cytometry and ELISA.

Western blot

Enriched mouse intestinal epithelial cells from Tfr1fl/fl control and Tfr1IEC-KO mice. Cells were lysed and sonicated in 150 mM Na chloride, 1.0% Triton X-100, and 50 mM Tris.

1:1,000 in PBST.

Anti-mouse HRP-linked IgG (GE Healthcare, NA931V) diluted 1:1,000 in PBST.

ECL Western Blotting Detection Kit (GE Healthcare, RPN2106).

The same clone (H68.4) is sold as Santa Cruz Biotechnology sc-65882; LifeSpan Biosciences LS-B2124; Invitrogen 13-6800, 13-6890.