This is a knockout-validated antibody summary, based on the publication "A validated antibody panel for the characterization of tau post-translational modifications", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Immunoblotting using hTg-Tau and Tau-KO brain lysates confirms the specificity for tau antibody. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: Tau (phospho S422)

Catalog number: ab79415

Summary: Rabbit monoclonal IgG against a synthetic peptide within human Tau phosphorylated on serine 422. Reacts with human by western blot. Unsuitable for immunoprecipitation, immunohistochemistry (paraffin) or flow cytometry.

Western blot

Brains from hTg-Tau and Tau-KO mice. Lysates were prepared by dounce homogenizer in 350 μl of lysis buffer containing 150 mM NaCl, 20 mM Tris pH 7.5, 1 mM EDTA, 1 mM EGTA, 1% Triton-X100 and protease, phosphatase, demethylase (500 μM IOX1, 2 μM Daminozide, 10 μM Paragyline Hydrochloride), deacetylase (10 μM Trichostatin A, 5 mM Nicotinamide), O-GlcNAcase (1 μM Thiamet-G) inhibitors.

5% BSA in 1X TBST.

1:250 dilution in blocking solution overnight at 4 °C.

1:20,000 dilution IRDye Donkey anti-mouse 800 or IRDye Goat anti-rabbit 680 in blocking solution for 2 h at room temperature.

Li-Cor Odyssey CLx imaging station.