This is a knockout-validated antibody summary, based on the publication "Tau-positive nuclear indentations in P301S tauopathy mice" [1] (discussed below) and "A validated antibody panel for the characterization of tau post-translational modifications

" [2] (see table 1 in the article for a summary of various Tau antibodies). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal

Company: ThermoFisher Scientific

Antibody: Tau

Catalog number: MN1000 (HT-7)

Summary: Mouse monoclonal antibody raised against purified human tau. Recognises an epitope between residues 159-163 of human tau.

Supplier recommended for WB, IP, ICC, IHC and elisa.

Note: this antibody does not recognise native mouse tau as the epitope is recognises is not conserved in mouse tau.

Immunohistochemistry

Paraformaldehyde-fixed cortex from the brains of wild-type and P301S transgenic mice.

PBS containing 1% BSA, 0.5% FCS and 0.3% Triton X-100.

Anti-tau antibody.

Avidin/biotin-based Elite Vectastain kit (Vector Laboratories).

Olympus BX41 microscope with Olympus DP70 camera.

h2 Figure

See Figure 2 from [1].