Tau antibody | knockout validation | ThermoFisher 13-6400

This is a knockout-validated antibody summary, based on the publication "Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Tau antibody | knockout validation | ThermoFisher 13-6400 figure 1
Figure 1. Cortical brain tissue from wild-type (WT) and tau knock-out (KO) mice were high-salt extracted and boiled where indicated. Samples were analyzed by immunoblotting using total tau (T49) antibody. From [1].
Antibody information

Mouse monoclonal IgG1

Company: ThermoFisher

Antibody: Tau

Catalog number: 13-6400

Summary: Mouse monoclonal IgG1 against bovine Tau. Reacts with bovine, chicken, human, mouse, rabbit, rat, and Xenopus. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry, flow cytometry and ELISA.

Validation Method

Western blot

Sample

Cells from 6-well culture dishes were scraped into 300 μl RIPA buffer (50 mM Tris pH 8.0, Whole cortex from wild-type (WT) and tau knock-out (KO) mice.

Primary incubation

1:1,000 dilution.

References
  1. Hwang A, Trzeciakiewicz H, Friedmann D, Yuan C, Marmorstein R, Lee V, et al. Conserved Lysine Acetylation within the Microtubule-Binding Domain Regulates MAP2/Tau Family Members. PLoS ONE. 2016;11:e0168913 pubmed publisher