Tau antibody | knockout validation | Sigma Aldrich T9450

This is a knockout-validated antibody summary, based on the publication "Impaired burrowing is the most prominent behavioral deficit of aging htau mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Tau antibody | knockout validation | Sigma Aldrich T9450 figure 1
Figure 1. Western blots of cortex homogenates of two-, four-, six-, nine- and fifteen-month-old C57Bl/6J (B6), mtau−/− (M) and htau (H) mice showing the absence of total tau (Tau-46) in mtau−/− mice. From [1].
Antibody information

Mouse monoclonal IgG1

Company: Sigma Aldrich

Antibody: Tau

Catalog number: T9450

Summary: Mouse monoclonal IgG1 against bovine Tau. Reacts with mouse, human, rat, and bovine. Suitable for western blot, immunohistochemistry, immunoprecipitation, ELISA and microarray.

Validation Method

Western blot

Sample

Control and mtau−/− mice cortices. Tissues were homogenized in 5× cold radioimmunoassay precipitation (RIPA) buffer plus inhibitors.

Blocking agent

5% milk in TBST (0.1% tween 20).

Primary incubation

1:500 dilution.

Secondary incubation

1:15,000 dilution IRDye 800CW Goat anti-Mouse (926-32210; LI-COR).

Detection

LI-COR Odyssey infrared scanner.

Clone note

The same clone (Tau46) is sold as BioLegend 806601; Cell Signaling Technology 4019; Sigma-Aldrich T9450.

References
  1. Geiszler P, Barron M, Pardon M. Impaired burrowing is the most prominent behavioral deficit of aging htau mice. Neuroscience. 2016;329:98-111 pubmed publisher