This is a knockout-validated antibody summary, based on the publication "Tau affects P53 function and cell fate during the DNA damage response", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot with Tau13, (loading control GAPDH), parental cells (wt) served as control. From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: Tau

Catalog number: sc-21796

Summary: Mouse monoclonal IgG1 κ against human recombinant Tau. Recommended for detection of all Tau isoforms of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin). Not inhibited by Tau phosphorylation.

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Figure 2. Immune staining was performed with Tau13 antibody and nuclear staining with DAPI. From [1].

Western blot | Immunocytochemistry

WT and Tau-KO SH-SY5Y neuroblastoma cells.

IF: 2 μg/mL secondary antibodies anti-mouse IgG-Alexa594 or -Alexa 488 (A-11032, A-11001, ThermoFisher Scientific).

IF: Fluorescence laser confocal microscopy (Nikon C2 microscope).

The same clone (Tau-13) is sold as BioLegend 835201, 835203, 835204; Santa Cruz Biotechnology sc-21796.