This is a knockout-validated antibody summary, based on the publication "Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. Western blot of Tau-1 in the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg mice, with n = 3 for each group. Normal anti-mouse (1) and mouse TrueBlot ULTRA (2) secondary antibodies were used to detect primary antibodies. The heat stable fraction was used to remove non-specificity (3). From [1].

Mouse monoclonal IgG1

Company: Invitrogen

Antibody: Tau

Catalog number: MN1020

Summary: Mouse monoclonal IgG1 against purified denatured bovine microtubule associated proteins. Reacts with human, and has been published to react also with dog, rabbit, rat, primate, fruit fly, hamster, and mouse. Suitable for western blot, immunohistochemistry, immunofluorescence and ELISA.

Western blot

Mouse brain cortex from TKO, 3xTg-AD, WT and hypothermic mice. Frozen cortices were homogenized in cold radioimmunoassay precipitation (RIPA) buffer (50 mM Tris-HCl, pH 7,4, 1% NP-40, 150 mM NaCl, 0,25% Na-Deoxycholate, 1 mM EDTA, 1 mM Na3VO4, 1 mM NaF, 1 mM PMSF and 10 ml/ml of proteases inhibitors P8340, Sigma Aldrich, Saint-Louis, MO, USA).

5% dry milk for 1 h at room temperature.

1/1,000 dilution overnight or 2 days (TrueBlot).

1/5,000 dilution goat anti-mouse Ig HRP (Jackson Immunoresearch Laboratories, 115-035-003) for 1h or 1/1,000 dilution TrueBlot ULTRA anti-mouse Ig HRP (eBiosciences, 18-8817-33) for 3 days.

ECL (Enhanced Chemiluminescence) detection kit (Millipore, Billerica, MA, USA) and visualized with a Fujifilm LAS4000 imaging system (Fujifilm Life Science USA, Stamford, CT).

Antibody shows high unspecificity with normal (HC+LC) anti-mouse Ig secondary antibodies, and TrueBlot or anti-LC secondary antibodies are necessary to detect tau signal.