This is a knockout-validated antibody summary, based on the publication "Specificity of anti-tau antibodies when analyzing mice models of Alzheimer's disease: problems and solutions", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of Tau-1 in the cortex of 3 mouse lines: control mice (WT and Hypothermic), Tau KO mice and 3xTg mice, with n = 3 for each group. Normal anti-mouse (1) and mouse TrueBlot ULTRA (2) secondary antibodies were used to detect primary antibodies. The heat stable fraction was used to remove non-specificity (3). From [1].

Mouse monoclonal IgG2a

Company: EMD Millipore

Antibody: Tau-1

Catalog number: MAB3420

Summary: Mouse monoclonal IgG2a against purified denatured bovine microtubule associated proteins. Reacts with bovine, rat and human. Suitable for western blot and immunohistochemistry.

Western blot

Mouse brain cortex from TKO, 3xTg-AD, WT and hypothermic mice. Frozen cortices were homogenized in cold radioimmunoassay precipitation (RIPA) buffer (50 mM Tris-HCl, pH 7,4, 1% NP-40, 150 mM NaCl, 0,25% Na-Deoxycholate, 1 mM EDTA, 1 mM Na3VO4, 1 mM NaF, 1 mM PMSF and 10 ml/ml of proteases inhibitors P8340, Sigma Aldrich, Saint-Louis, MO, USA).

5% dry milk for 1 h at room temperature.

1/1,000 dilution overnight.

1/5,000 dilution goat anti-mouse Ig HRP (Jackson Immunoresearch Laboratories, 115-035-003) or 1/1,000 dilution TrueBlot ULTRA anti-mouse Ig HRP (eBiosciences, 18-8817-33) for 1h.

ECL (Enhanced Chemiluminescence) detection kit (Millipore, Billerica, MA, USA) and visualized with a Fujifilm LAS4000 imaging system (Fujifilm Life Science USA, Stamford, CT).