This is a knockout-validated antibody summary, based on the publication "Characterization of Dopaminergic System in the Striatum of Young Adult Park2-/- Knockout Rats", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG3 (kappa light chain)
Company: Santa Cruz
Antibody: TaR
Catalog number: sc-398096
Summary: Mouse monoclonal IgG3 (kappa light chain) against amino acids 181-247 mapping near the C-terminus of mouse TaR-1. Recommended for detection of TaR-1 of mouse and rat origin by western blot, immunoprecipitation, immunofluorescence and ELISA.
Immunohistochemistry
Brain tissues from WT and TAAR1−/− knockout rats.
1:100 dilution overnight at 4 °C in the blocking buffer.
Alexa Fluor-488 conjugated secondary antibody (Invitrogen, Carlsbad, CA, USA) for 2.5 h at room temperature.
Leica TCS SPE-II laser scanning confocal microscope (Leica, Wetzlar, Germany).
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.