TXNDC11 antibody | knockout validation | Abcam ab188329
DOI
//dx.doi.org/10.13070/ko.en.7.2187
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

TXNDC11 antibody | knockout validation | Abcam ab188329 figure 1
Figure 1. Immunoblot of TXNDC11 in wild-type and two independent TXNDC11-deficient KBM7 cell clones. Beta-actin was used as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Abcam

Antibody: TXNDC11

Catalog number: ab188329

Summary: Rabbit monoclonal IgG against a recombinant fragment within human TXNDC11 aa 850 to the C-terminus. Reacts with: human, mouse, and rat. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control wild-type and TXNDC11-KO KBM7 cells. Cells were lysed in 1% digitonin in TBS plus 10 mM iodoacetamide (IAA), 0.5 mM PMSF on ice for 30 min.

Blocking agent

5% milk in PBS+0.2% Tween-20.

Primary incubation

1:5,000 dilution.

Detection

West Pico or West Dura (Thermo Fisher Scientific).

References
  1. Timms R, Menzies S, Tchasovnikarova I, Christensen L, Williamson J, Antrobus R, et al. Genetic dissection of mammalian ERAD through comparative haploid and CRISPR forward genetic screens. Nat Commun. 2016;7:11786 pubmed publisher