This is a knockout-validated antibody summary, based on the publication "Spadin Modulates Astrocytic Passive Conductance via Inhibition of TWIK-1/TREK-1 Heterodimeric Channels", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Immunocytochemical images of control gRNA (Con gRNA) or TWIK-1 gRNA transfected cultured astrocytes stained with anti-TWIK-1 antibody. From [1].

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: TWIK-1

Catalog number: sc-517040

Summary: Mouse monoclonal antibody against amino acids 265-336 representing partial length human TWIK-1. Recommended for detection of TWIK-1 of mouse, rat and human origin by western blot, immunoprecipitation and ELISA.

Immunocytochemistry

Control and TWIK-1 KO cultured astrocytes.

0.1% Triton X-100 and 3% BSA for 1 h.

1:100 dilution at 4 °C overnight.

1:400 dilution Alexa Fluor 594-conjugated secondary antibodies (Jackson Immunoresearch, West Grove, PA, USA) for 1 h at room temperature.

Confocal microscopy using a Nikon A1 confocal microscope (Nikon Instruments Inc., Melville, NY, USA).