TWEAKR Fn14 antibody | knockout validation | Epitomics ab109365
DOI
//dx.doi.org/10.13070/ko.en.7.2176
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "Neuropsychiatric disease in murine lupus is dependent on the TWEAK/Fn14 pathway", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Epitomics

Antibody: TWEAKR Fn14

Catalog number: ab109365

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human TWEAKR Fn14 (internal sequence). Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation, and immunohistochemistry (paraffin and frozen). Unsuitable for immunocytochemistry.

Validation Method

Immunohistochemistry

Sample

MRL/lpr Fn14 wild type (WT) and knockout (KO) mice brains. Tissues were fixed in formalin fixed and embedded in paraffin for sectioning.

Secondary incubation

DakoCytomation Envision + Dual Link System-HRP kit (Dako, Carpinteria, CA), following the manufacturer’s instructions.

Detection

3,3-diaminobenzidine chromogen solution and Zeiss AxioObserver microscope.

Figure

Please see Figure 1b in the article [1].

References
  1. Wen J, Xia Y, Stock A, Michaelson J, Burkly L, Gulinello M, et al. Neuropsychiatric disease in murine lupus is dependent on the TWEAK/Fn14 pathway. J Autoimmun. 2013;43:44-54 pubmed publisher